|
KMID : 1094720160210040477
|
|
Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 4 p.477 ~ p.482
|
|
|
Reconstruction of methanol and formate metabolic pathway in non-native host for biosynthesis of chemicals and biofuels
|
|
Nguyen Anh Duc
Hwang In-Yeub
Chan Jeon-Young
Lee Eun-Yeol
|
|
|
Abstract
|
|
|
|
One-carbon feedstock such as methanol and formate has attracted much attention as carbon substrate of industrial biotechnology for production of value-added chemicals and biofuels. Productivity improvement of natural one-carbon metabolic pathways in native hosts such as methanotrophs is somewhat difficult due to inefficient genetic tools and low specific growth rate. As an alternative, metabolic engineering can create new and efficient metabolic pathways of one-carbon substrate that can be readily transferred to non-native hosts. In this paper, recent progresses in protein and metabolic engineering for creation of methanol and formate-utilizing synthetic pathways based on RuMP cycle and formolase are reviewed. Perspectives on one-carbon metabolic pathway engineering in non-native host are also discussed.
|
|
|
KEYWORD
|
|
|
methanol, formate, formolase, methanol dehydrogenase, RuMP cycle
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|